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Excellent Anti Spyware used by PC repair people etc. Just download to your PC or usb drive, CD etc. It will save as a random name to avoid malware recognizing it. Then choose your scanning options.
Also you can download the latest definitions for SuperAntiSpyware. This is an executable just click and it will update SuperAntiSpyware on your machine if it has already been installed (not usb version).

Norman Malware Cleaner:

Norman Malware Cleaner, a Malware Cleaner that can be run directly from a USB Drive or other portable device.

Dr.WEB CureIt – Stand Alone AntiVirus Utility:

This stand-alone works directly from USB drive and can detect and removal all the common versions of malware.

F-Secure Easy Clean:

A technology by F-Secure; a large download but includes a large database of definitions. This does not seem to be known as a direct from usb application; but the .exe does run directly from usb. They state for best results run this application in Safe Mode with Networking.

F-Secure BlackLight – RootKit Eliminator:

Free rootkit detection and removal software that works directly from your USB drive.

HijackThis – Portable Malware Detection and Removal:

HijackThis is available as a stand alone executable, working as a good Anti Malware detection tool to join your collection.

CWShredder – Portable AntiSpyware:

Can be used for detection and removal of all traces of the CoolWebSearch clan of Spyware. CoolWebSearch is a browser hijacker.

AVZ Antiviral Toolkit 4.32:

AVZ Antiviral Toolkit is a powerful freeware application that deletes many brands of malware. This is a part of Kaspersky Labs straight from your USB stick.

AppRemover - Antivirus and AntiSpyware Removal Tool

AppRemover is a Portable program that removes old and new Anti Virus and Anti Spyware programs that are giving problems

For more on USB applications check out